解剖学报

解剖学报 ›› 2014, Vol. 45 ›› Issue (3): 304-309.doi: 10.3969/j.issn.0529-1356. 2014.03.002

• 神经生物学 • 上一篇    下一篇

节制饮食增强猫视皮层γ-氨基丁酸和脑源性神经营养因子的表达

陈翠云 朱慧 李鹏 孙庆艳 华田苗*   

  1. 安徽师范大学生命科学学院，安徽 芜湖 241000
  • 收稿日期:2013-11-18 修回日期:2013-12-23 出版日期:2014-06-06 发布日期:2014-06-06
  • 通讯作者: 华田苗 E-mail:tmhua@mail.ahnu.edu.cn
  • 基金资助:

    Delta原钙黏蛋白（delta-protocadherin）在脊髓发育过程中的表达和功能调控研究;安徽省教育厅重点项目

Dietary restriction enhances γ-aminobutyric acid and brain derived neurotrophic factor expression in the visual cortex of the cat

CHEN Cui-yun  ZHU Hui  LI Peng  SUN Qing-yan  HUA Tian-miao*   

  1. College of Life Sciences, Anhui Normal University, Anhui Wuhu 241000, China
  • Received:2013-11-18 Revised:2013-12-23 Online:2014-06-06 Published:2014-06-06
  • Contact: HUA Tian-miao E-mail:tmhua@mail.ahnu.edu.cn

摘要：

目的 探讨节制饮食对猫初级视皮层内抑制性神经递质γ-氨基丁酸（GABA）和脑源性神经营养因子（BDNF）表达的影响。方法 Nissl染色示初级视皮层分层并用于神经元计数; 免疫组织化学方法标记6只猫GABA和BDNF免疫阳性神经元。切片于Olympus光学显微镜下观察摄片，用Image-Pro Express 6.0软件进行细胞计数和免疫阳性反应的吸光度值统计。结果 与正常饮食对照猫相比, 节制饮食组猫的初级视皮层神经元平均密度无显著变化, GABA 免疫阳性神经元密度显著增大, 阳性反应的平均吸光度值显著升高; BDNF免疫阳性细胞的密度和阳性反应的平均吸光度值亦明显增大。结论 节制饮食能显著增强猫视皮层内GABA和BDNF的表达，这可能对视皮层神经元的结构和功能有保护作用，从而延缓衰老进程。

关键词: 节制饮食, γ-氨基丁酸, 脑源性神经营养因子, 视皮层, 免疫组织化学, 猫

Abstract:

Objective To explore the effect of DR on the expression of inhibitory neurotransmitter, γ-aminobutyric acid (GABA) and brain derived neurotrophic factor (BDNF) in the primary visual cortex of young cats. Methods Totally 6 cats were wsed in this study. Nissl staining was used for cortical layer identification and cell counting. Immunohistochemical techniques were utilized to label GABA- and BDNF-positive neurons. Sections were observed under an Olympus light microscope and photographed with a digital camera. The cell density and absorbance of immunoreactivity were measured with Image-Pro Express 6.0 softwares.
Results Our results showed that the mean density of Nissl-stained neurons in the primary visual cortex (V1) of the DR group showed no significant difference from that of the control group. However, the mean density of GABA-immunoreactive neurons in each cortical layer of V1 in DR cats was significantly higher than that in control cats. The mean immunoreactive intensity of GABA-positive neurons, as indicated by the average absorbance, increased significantly in DR cats relative to control ones. Accompanied with the elevation of GABA expression, DR enhanced BDNF expression in V1, as indicated by an increased mean density of BDNF-positive neurons and BDNF-immunoreactive average absorbance in DR cats relative to controls. Conclusion These results indicate that dietary restriction leads to a concurrent expression upregulation of GABA and BDNF, which may likely compensate for brain functional degradation during senescence and thus delay aging process.

Key words: Dietary restriction, γ-Aminobutyric acid, Brain derived neurotrophic factor, Visual cortex, Immunohistochemistry, Cat